一株新轮状病毒引起河北省石家庄市成人腹泻爆发流行

A Novel Rotavirus Causing Large Scale of Adult Diarrhea in Shi Jiazhuang

1997年 4月 10～ 2 8日 ,在河北省石家庄市某高校发生一起成人急性腹泻的爆发流行 ,累积10 0 0多名大学生发病。经不连续聚丙烯酰胺凝胶 (PAGE)电泳检查腹泻病人粪便标本核酸图谱一致呈4 - 2 - 1- 1- 1- 1- 1排列的新轮状病毒 (新RV) 14份 ,阳性率占 4 7% (共检标本 30份 ) ,且未见其它带型的轮状病毒。提示该核酸图谱的轮状病毒是此次成人急性腹泻爆发流行的主要病原。用已知ADRV第 5及第 9基因片段末端引物进行逆转录 -聚合酶链反应 (RT -PCR) ,结果阳性对照ADRV的扩增结果都为阳性 ,而新RV的扩增结果都为阴性。这些结果表明该新RV不属于B组轮状病毒 ,是与成人腹泻轮状病毒 (ADRV)完全不同的新RV。非ADRV的轮状病毒引起成人腹泻爆发流行在河北省尚属首次。

An outbreak of adult diarrhea causing more than 1 000 hospitalized cases among graduates in a university in Shi Jiazhuang city (Hebei province),occurred in 10-28,April,1997. The non-bacterial pathogenic agent is confirmed as Rotavirus first by PAGE. Forteen samples have been demonstrated sharing the same nucleic acid electrophoresis pattern of 4-2-1-1-1-1-1. The positive rate was 47% (thirty samples have been checked) with no other RV pattern. Dada showed that RV with this kind of nucleic acid electrophoresis pattern were responsible for the outbreaks acute diarrhea in adults. The novel RV and known ADRVwas RT-PCR with end-primers 5, 9 of typical ADRV, the positive control typical ADRV's results are positive, the novel RV's are negative. Experimental results showed that the novel RV did not belong to group B RV, totally different from all the known ARDV. This case was the first reported one in Hebei province with an outbreak of adult diarrhea not by any known ADRV.