摘要
目的:探讨曲古菌素A(TSA)对食管癌EC1细胞凋亡的影响。方法:用DMSO(对照组)和0.3、0.5及1.0μmol/L的TSA处理EC1细胞24 h,用Annexin V-FITC和PI双染色,流式细胞仪检测4组细胞的凋亡率,West-ern Blot检测4组细胞中Bax及Bcl-2表达的变化。结果:对照组、0.3、0.5及1.0μmol/L TSA组作用24 h后,EC1细胞凋亡率分别为(2.18±0.37)%、(2.96±0.34)%、(7.41±1.01)%和(14.03±1.23)%,Bax蛋白表达水平分别为(0.42±0.23)、(0.45±0.11)、(1.16±0.28)和(1.89±0.30),Bcl-2蛋白表达水平分别为(1.15±0.14)、(1.11±0.15)、(0.59±0.06)和(0.40±0.07),4组间3指标差异均有统计学意义(F=211.96,25.12和33.68,P均<0.001),随TSA作用浓度的增加,EC1细胞凋亡率、Bax蛋白表达均增加,Bcl-2表达减少。结论:一定剂量的TSA可以诱导EC1细胞凋亡,凋亡的发生可能与Bax表达增加和Bcl-2表达减少有关。
Aim: To explore the effects of trichostatin A on apoptosis in EC1 cells. Methods:EC1 cells at logarithmic growth phase were treated with O. 3 μmol/L, 0.5 μmol/L and 1.0 μmol/L TSA for 24 h, the cells were stained with Annexin V-FITC and PI for analyzing apoptosis by flow cytometry, the expressions of Bax and Bcl-2 were investigated with Western Blot. Cells treated with DMSO were the control. Results: Apoptosis rates of O. 3 μmol/L,0.5 μmol/L, 1.0 μmol/L trichosta- tin A groups and the control group were (2.96 -±0.34)% ,(7.41 ±1.01)% , (14.03 ±1.23)% and (2.18 ±0.37)% ;the Bax expression level was (0.45 ± 0.11 ) , ( 1.16 ± 0.28 ) , ( 1.89 ± 0.30) and (0.42 ± 0.23 ) ; the Bcl-2 expression level was (1.11 ±0.15),(0.59±0.06),(0.40±0.07), and (1.15 ±0.14). There were significant differences in the 3 indexes among the 4 groups (F = 211.96,25.12, and 33.68, all P 〈 O. 001 ). Apoptosis rates and the Bax expression level increased and the Bel-2 expression level decreased with the increase of the dosage of TSA. Conclusion : TSA is able to induce apoptosis of esophageal carcinoma cell line EC1 cells partly through the regulation of the expression of Bax and Bcl-2.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2010年第2期180-182,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
教育部科技研究重大项目207150