微卫星不稳定性在大肠癌发生中的作用及其与预后的意义

Role of microsatellite instability in occurrence of colorectal carcinoma and its prognostic significance

目的：探讨微卫星不稳定性在大肠癌发生中的作用及其与大肠癌预后的关系。方法：采用ＰＣＲ－ＳＳＬＰ法对５６例大肠癌Ｄ１８Ｓ３４，Ｄ１７Ｓ７９９，Ｄ５Ｓ４０９，ＴＣＦ－２，ｐ５３和Ｒｂ６个位点微卫星不稳定性进行了检测。结果：显示大肠癌ＭＳＩ总阳性率４４．６４％，其中，ＨＰＮＣＣ≥２个位点ＭＳＩ阳性率７５．００％，散发性大肠癌≥２个位点阳性率２８．８５％。ＭＳＩ阳性大肠癌患者３、５年生存率高于ＭＳＩ阴性患者。结论：ＭＳＩ可能为大肠癌发生的又一种分子机制。ＭＳＩ阳性大肠癌生物学行为较好，预后较佳。

Objective: To explore the role of microsatellite instability (MSI) in the occurrence of colorectal carcinoma and the relationship between MSI and prognosis of the carcinoma. Methods: MSI at 6 loci of D18S34, D17S799, D5S409, TCF 2, P53 and Rb in 56 cases of colorectal carcinoma were detected with RCR SSLP. Results: The total positive rate of MSI was 44.64% . The 3 or 5 year survival rate was higher in the cases with MSI than in those without. Conclusion: MSI may be another molecular mechanism of occurrence of colorectal carcinoma. The cases of colorectal carcinoma with MSI have better biological behavior and outcome. Therefore, the detection of MSI might be useful for prediction of the prognosis of colorectal carcinoma.