摘要
目的:探讨海马CA1区氨基丁酸A受体(GABAAR)、氨基丁酸B受体(GABABR)活动对P3波的影响机制。方法:10只健康家兔,用听Obdbal方案,被动条件记录Pz点P3波。导管埋植术后,采用海马CA1区微量注入GABAAR选择性拮抗剂Bicuculine,GABABR激动剂Baclofen,观察干预GABA受体亚型对兔P3波的影响。结果:Bicuculine(3、6、12μmol/L)呈一定量效依赖衰减P3b波电压振幅,Baclofen(6.25,12.5,25mmol/L)呈明显量效依赖延长、阻抑P3b波。外源性GABA(100mmol/L)可翻转Bicuculine(12μmol/L)、Baclofen(25mmol/L)对P3b波振幅的抑制,但其潜伏期延长。结论:海马CA1区GABAARIPSP(Inhibitorypostsynapticpotential)可能即是P3b振幅的重要组成。
Objective: To explore the mechanism for the effects of GABAAR and GABABR on P3 potentials in CA1 region. Methods: P3 potentials at Pz site were recorded in 10 healthy rabbits. After the catheter was implanted, either Bicuculline, a selective antagonist to GABAAR, or Baclofen, an agonist to GABABR, was microinfused into the CA1 region to observe the effects of intervention of GABA subtypes on P3 potentials. Results: P3 amplitude was markedly decreased by Bicuculline in a dose dependent manner (3, 6, 12 μmol/L) and P3 latency was increased by Baclofen in the same manner (6.25, 12.5, 25 mmol/L). The blocking effects of Bicuculline (12 μmol/L) or Baclofen (25 mmol/L) on P3 potentials could be reversed by microinfusion of GABA (100 mmol/L), but the latency was prolonged. Conclusion: GABAAR inhibitory postsynaptic potential in the CA1 region might be one important component of P3b amplitude and GABAR action can prolong P3b latency through reducing capacity of neurons to form P3b potential.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第6期508-511,共4页
Journal of Third Military Medical University