去神经支配对大鼠创面愈合的影响

Effect of denervation on skin wound healing in rats

目的通过制备去神经支配大鼠创面模型,观察去神经大鼠创面愈合率、创面血管和皮肤增殖改变情况,为去神经创面Ⅹ合机制提供理论依据。方法分离并切断SD大鼠胸9～腰1右侧脊神经,造模后在背部对称制作两个直径为1.0cm的圆形创面,分别观察两侧创面愈合时间、创面大体和组织形态学、创面细胞增殖活性、创面修复过程中血管再生情况,并对数据进行统计学分析。结果实验组愈合时间在4周,对照组为3周;实验组创面上皮化速度慢于对照组;实验组愈合表皮层较薄,上皮细胞大小不一;愈合时真皮和表皮层细胞增殖活跃,2～4周时实验组增殖活性低于对照组(P<0.05);创面微血管主要分布于真皮层,密度随Ⅹ合时间增加,但2～4周实验组表现较对照组弱(P<0.05)。结论去神经后皮肤创面细胞增殖低下,愈合时间延长,血管再生现象受到抑制。

Objective To observe the effect of denervation on wound healing rate, cell proliferation activity and vascular regeneration. Methods Right spinal nerves of T9 to L1 of were isolated and dissected on forty Spraque-Dawley（SD） rats. Subsequently, 1.0cm-diameter round full-thickness cutaneous wounds were made symmetrically within the denervated area on the right side of the back（experimental group） and the normal innervated area on the left side（control group）. Wounds were measured and harvested at 1,2,3,4 weeks. Wound healing rate, histology and morphometry, cell proliferation actiqity and vascular regeneration were analyzed over time. Results The wounds of control group and experimental group were completely heal on 3rd and 4th week respectively. Epithelization of control group appeared earlier and faster than experimental group, wound healing rate of experimental group were decreased significantly at 2nd and 3rd week （ P〈0.05 ）as well. The cell proliferation activity of wounds surface were higher in dermis and epidermis. Furthermore, cell proliferation activity of experimental group were weaker than control group during 2 to 4 weeks （ P〈0.05 ）. Regenerated wound capillary vessel laied in dermis mainly. Wound capillary vessel density was lower significantly in the experimental group （P〈0.05） at 2 to 4 weeks when compared with the controls. Conclusion Loss of innervation on skin may be responsible for the cell proliferation activity hypoproliferation, wound healing delay and vascular regeneration depression.