限制性液体复苏对失血性休克大鼠网状内皮系统的影响

Effects of restricted fluid resuscitation on reticuloendothelial system in rats with hemorrhagic shock

目的探讨限制性液体复苏对失血性休克大鼠网状内皮系统的影响。方法60只SD大鼠制成未控制性重度失血性休克模型,随机分成对照组、NF组(无液体复苏组)、NS40组(限制性液体复苏组)和NS80组(常规大量液体复苏组),检测和比较休克复苏后各组存活大鼠肝脏枯否细胞和腹腔巨噬细胞的吞噬功能。结果重度失血性休克大鼠失血后150min存活率NF组、NS40组和NS80组比对照组明显提高,NS40组较NS80组显著改善(P<0.05);NS40组大鼠肝脏枯否细胞和腹腔巨噬细胞的吞噬功能较NS80组明显改善(P<0.05)。结论限制性液体复苏可以显著改善失血性休克大鼠的网状内皮系统的吞噬功能,提高大鼠的免疫功能,降低死亡率。

Objective To investigate the effects of restricted fluid resuscitation on reticuloendothelial system in rats with hemorrhagic shock. Methods A model of rat with severe hemorrhagic shock caused by active bleeding was established in 60 SD rats. Those rats were randomly divided into control group, NF group（no fluid resuscitation group）, NS40 group（restricted fluid resuscitation group） and NS80 group（conventional large_volume fluid resuscitation group）, After different treatments, survival rats in each group were detected and compared ofthe phago- cytosis function of the Kupffer cell and the peritoneal macrophage. Results Restricted fluid resuscitation could reduce the mortality rate of rat in shock stage significantly（P〈0.05）. When compared with NS40 and NS80 group, phagocytosis function of the Kupffer cell and the peritoneal macrophage in NS40 group rats was obviously increased （P〈0.05）. Conclusion Restricted fluid resuscitation can significantly improve the phagocytic function of the Kupffer cell and the peritoneal macrophage in hemorrhagic shock rat, enhance the immune function ofrat to reduce the mortality.