He-Ne激光照射对离体小鼠巨噬细胞膜电位的影响

Effect of the Cellular membrane Potential on the Mice Macrophagic Cells with helium-neon Laser Irradiated in Vitro

目的：低功率Ｈｅ—Ｎｅ激光照射引起细胞膜电位的改变，为激光照射治疗提供理论依据。方法：不同能量密度的Ｈｅ—Ｎｅ激光照射离体小鼠巨噬细胞，用图像分析系统检测Ｃｙａｎｉｎｅ荧光染色的巨噬细胞膜电位的变化。结果：用光剂量１．２３９—２．４７８Ｊ／ｃｍ２）的Ｈｅ—Ｎｅ激光照射巨噬细胞，与对照组相比，照射组细胞面积增大，细胞膜电位荧光强度显著增强。照射２０ｍｉｎ，强度为１５０．６７±４９．２５（任意单位），对照组为１０７．００±３３．４９。结论：适当强度的Ｈｅ—Ｎｅ激光照射，可使巨噬细胞超极化，细胞激活，功能增强。

Objective:A changs of the cellular membrane potential was induced by the low power laser irradiation. In order to offer in accordance with a theory is provided by the laser irradiated therapy. Methods:The mice macrophagus were irradiated with different intensity He_Ne laser in vitro. The change of the cellular membrane potential on the macrophage was detected by cyanine stained with image analysis system. Results:In comparison with the control, with the He_Ne laser of the light dose of 1.239-2.478 J/cm 2 were irradiated the macrophagus which areas increased and the fluorescent density heightened significantly in the irradiation groups. After the laser irradiation 20 min, the laser intensity was 150.67±49.25(arbitrary unit) and the control was 107.00±33.49. Conclusion: The hyperpolarization of the macrophagus was induced by the proper intensity of the He-Ne laser irradiation, which was activated the cells and function strengthened.