干旱地区几种豆科植物根瘤菌的数值分类和SDS-PAGE全细胞蛋白电泳分析

SDS PAGE ANALYSIS OF WHOLE CELL PROTEIN AND NUMERICAL TAXONOMY OF Rhizobia OF SOME LEGUMES GROWING IN ARID REGION

从新疆、内蒙干旱地区苜蓿属、草木樨属、锦鸡儿属植物的根瘤中分离出５４株根瘤菌，对其中４８株根瘤菌和２２株参比菌一起进行了数值分类研究，在８５％的相似性水平上，未知菌分为３个不同于已知种的新群．另加入６株分离自锦鸡儿属植物的根瘤菌，共７６株菌进行了全细胞蛋白ＳＤＳＰＡＧＥ分析，在８０％的相似性水平上，已知菌相应成群，未知菌除ＸＪ９６３４２在６８％的相似性水平上和群５、６、７、８、Ｒ．ｇａｌｅｇａ聚群外，其余未知菌则分布在４、５、６、７、８、９、１０七个群中．群４中所有菌株属于数值分类的群１；群６中有３株菌属于数值分类的群３；群９中有８株菌为数值分类群２的菌株．反映了两种方法在分类上得到的结果比较近似．

strains of Rhizobia were isolated from root nodules of Medicago , Melilotus ,and Caragana , and 48 strains were numerically classified in comparison with 22 reference strains of Rhizobia . At the similarity level of 85%, the unknown strains were divided into 3 new groups different from the reference strains.76 strains (including 70 strains used in numerical taxonomy, 6 strains isolated from Caragana in Xinjiang recently ) were analyzed by SDS PAGE of the whole cell protein. At the similarity level of 80%,the known strains were clustered into independent groups;the unknown strains were located in group 4,5,6,7,8,9,10 except that XJ96342 were clustered with group 5,6,7,8, R.galegae at the similarity level of 68%. All strains of group 4 were located in group 1 in the numerical taxonomy; 3 strains of group 6 were located in group 3 in the numerical taxonomy; 8 strains of group 9 were located in group 2 in the numerical taxonomy.These results show that there are some consistencies between the numerical taxonomy and SDS PAGE analysis of the whole cell protein in taxonomy .