蚊体内间日疟原虫SSUrDNA特定片段的克隆与鉴定

COLONING AND IDENTIFICATION OF SPECIFIC SSUrDNA FRAGMENT OF PLASMODIUM VIVAX IN MOSQUTTO

利用套式ＰＣＲ技术体外扩增蚊体内间日疟原虫ＳＳＵｒＤＮＡ长１２１ｂｐ的特定片段，将此片段克隆到载体ＰＵＣ１８中，转化受体菌株ＤＨ５α，测序结果表明，扩增ＤＮＡ序列与背景序列完全一致，证实为间日疟原虫ＳＳＵｒＤＮＡ目的片段。

The specific SSUrDNA fragments of 121bp from DNA extracted from Plasmodium vivax infected mosquito were amplified in vitro using nested polymerase chain reaction. The products of nested PCR were coloned in SmaI site of PUC18. The result of sequencing showed that the sequence of amplified DNA fragment was indeed SSUrDNA of P.vivax.