猕猴桃属(Actinidia)植物的离体种质保存

In vitro conservation of Actinidia

本文研究了猕猴桃属植物离体种质资源保存的方法。在一年中于盛夏时采取的植物材料在离体培养时能得到最好的效果；改进的剥离茎尖的方法使污染率大大降低，在ＭＳ附加ＢＡ０．５，Ｚ０．１，ＧＡ０．１～０．５ｍｇ／Ｌ，蔗糖３％，琼脂０．５５％的培养基中茎尖生长良好并且不产生不定芽。通过茎尖培养方法已保存了１０多个种，４０多个猕猴桃的离体种质。

This paper deals with the methods for in vitro conservation of Actinidia. Early summer was the best time to obtain the plant materials.The contamination rate was greatly decreased by using a improved technology to excise the shoot tips and the shoot tips grew well and had no adventitious shoot formation in MS medium supplemented with BA 0.5, Z 0.1, GA 0.1～0.5 mg/L.Up to date, about 12 species and more than 40 clones of Actinidia plants have been conserved in vitro.