不同发育时期柞蚕雌蛾粘液腺的组织形态学观察

Histomorphology of Antheraea pernyi Colleterial Glands at Different Developmental Stages

【目的】对柞蚕雌蛾粘液腺的生长发育过程和组织结构特征进行研究,并分析其内容物成分,为阐明粘液腺分泌胶着物质的机理提供参考。【方法】通过解剖获得不同发育时期的粘液腺并制备组织石蜡切片,观察其形态特征及组织显微结构。【结果】随着发育阶段的推进,粘液腺形态呈现出逐渐增大趋势,至羽化前达最大。而粘液腺内容物的颜色亦发生了显著变化,由透明色逐渐变为深黑色。光学显微镜观察结果显示,粘液腺的分泌部和贮藏部横截面结构存在差异,前者具备分泌细胞的特征,而后者不具备。【结论】粘液腺的形态结构特征在不同发育时期及组织部位存在差异。

[Objective] The experiment on morphological characteristics and microstructure of colleterial glands from Antheraea pernyi female silkmoths at different developmental stages was carried out to investigate the secretion mechanism of glue materials in the colleterial glands. [Method] The morphological characteristics and microstructure of colleterial glands were observed by anatomy and tissue paraffin section at different developmental stages and tissues. [Result] The colleterial glands became bigger with the development and reached the peak as the silkmoth emerges while the color of its inclusion was changed remarkably from transparent to blue black. Differences were observed in the microstructure between secretion section and reservoir section, the former showed the characteristics of secreting cells while the later did not. [Conclusion] Differences in morphological characteristics and microstructure of colleterial glands were observed at various stages and in different tissues.