中华按蚊幼虫肠壁膜泡的制备及其鼠抗血清对幼虫的作用

Preparation of Gut Cell Membrane Fraction of Anopheles sinensis Larvae and the Effect of Its Mouse Antisera on the Larvae

目的：搞清Ｂ．ｓ杀虫蛋白对蚊幼虫肠细胞膜的作用机理，从而改进杀虫蛋白活性。方法：差速离心制备肠细胞膜泡，ＳＤＳ－ＰＡＧＥ电泳分析其蛋白组分；测试其鼠抗血清对蚊幼虫的效应。结果：膜制备物蛋白组分分析显示了膜泡的纯度，而抗血清则对Ⅰ龄幼虫有明显的致死效应；日龄越小，致死率越高。

The toxicity of some bacillus larvicidal proteins depends on their binding affinity with larval gut cell membrane. Preparation of membrane vesicle of mosquito larvae gut was essential to research on the larvicidal mechanism and to improvement of the larvicidal proteins by protein engineering methods. In this study, we prepared and analysed larval mosquito gut cell membrane fraction. While, we prepared its mouse antisera and observed the biological effect of the antisera on mosquito larvae. The results showed that the main protein band of the membrane vesicle was that of 51kDa and the larval mortality caused by the antisera was obviously higher than that by the control sera. This suggested that the antibody components indeed have binding activity in the midgut.