PCR和分离培养同时检测儿童呼吸道感染的支原体

DETECTION OF MYCOPLASMAS IN THROAT SWABS FROM CHILDREN WITH RESPIRATORY TRACT INFECTIONS BY PCR AND ISOLATION

为了解广州市儿童呼吸道支原体感染情况，用一条共同的上游引物，二条特异性的下游引物建立的ＰＣＲ方法能同时扩增ＭＰ的６９１ｂｐ和ＭＧ的４３８ｂｐ粘附因子基因片段，但不会扩增其他支原体和细菌的ＤＮＡ。以ＰＣＲ和分离培养同时检测了１６２份呼吸道感染患儿的咽拭标本，结果ＰＣＲ检测到１２份阳性ＭＰＤＮＡ，阳性率为７４％；从１份阳性标本分离培养并克隆出１株ＭＰ。二方法均未检测到ＭＧ。提示在广州市呼吸道感染患儿中存在ＭＰ的感染，所检测的患儿无ＭＧ感染。

To study the prevalence of mycoplasma infection in the respiratory tract of children in Guangzhou. PCR technique was established for the amplification of 691bp and 438bp oligonucleotides from Mycoplasma pneumoniae and Mycoplasma genitalium adhesin gene respectively by using a common upstream primenr and two species specific downstream primers. DNA from other mycoplasmas and bacteria did not give rise to specific amplification products. Throat swabs from 162 children with respiratory infections were analyzed by PCR and microbiological culture. Twelve of them (7 4%) were found positive for M. pneumoniae by PCR, one M. pneumoniae strain was isolated from one of these 12 samples by culture. All samples were negative for M. genitalium by PCR and culture. The results indicate that M. pneumoniae infections were present in children in Guangzhou and M. genitalium infection was not found among children who were examinated.