橡胶树冷应答转录组cDNA-AFLP分析

cDNA-AFLP Analysis on Transcriptomics of Hevea brasiliensis Induced by Cold Stress

以橡胶树11个抗寒无性系和12个不抗寒无性系为研究对象,利用cDNA-AFLP技术对23个橡胶树无性系低温处理前后的基因表达谱进行差异分析。结果表明:通过100对引物组合的扩增筛选,共获得12条在2组材料中表现一致的差异片段(GenBank登录号:GO269543-GO269554);BLASTn和BLASTx比对分析显示,TDF7-8,TDF5-15,TDF16-103条片段分别与植物抗逆相关的ABC转运蛋白、3-脱氧-D-阿拉伯庚酮糖-7-磷酸合成酶(DAHPS)、WRKY基因有较高相似性,其余9条片段在GenBank未比对到同源序列。

Rubber tree （Hevea brasiliensis） is the main source of nature rubber. Although rubber planting industry has become one of the important industries of Chinese agriculture in tropical areas,low temperature stress still restricts the development of rubber planting industry in China. The differential transcription of 11 cold-resistant rubber clones and 12 cold susceptible rubber clones was analyzed with and without cold stress by cDNA-AFLP. Through screening 100 primer sets,a total of 12 specific fragments were identified,cloned and sequenced,whose GenBank accession No. was GO269543 - GO269554 respectively. By aligning with BLASTn and BLASTx,three fragments,TDF7-8,TDF5-15 and TDF16-10,exhibited the high homology with the resistance related gene ABC transport protein family,3-deoxy-D-arabino- heptulosonate 7-phosphate synthase （ DHAPS ） and WRKY genes,respectively. All of the three fragments were up- regulated under cold stress. The other 9 fragments didn’t have high homologous sequences in GenBank. The results would be useful knowledge for rubber cold-resistant molecular breeding in future.