大鼠肝细胞癌变过程中热休克蛋白gp96的异常表达及变化特征

Character of Expression and Alteration of Rat Heat Shock Protein gp96 During Development of Hepatocellular Carcinoma

目的探讨大鼠肝细胞癌变过程中热休克蛋白(HSP)gp96的表达与变化特征。方法以2-乙酰氨基芴(2-FAA)喂饲SD大鼠诱发肝癌,以免疫组化染色及Western blot观察大鼠肝组织中gp96蛋白的定位及表达水平。结果SD大鼠在喂饲2-FAA后,在诱癌早期肝细胞发生颗粒样变性,中期出现不典型增生,后期见大量癌巢结节。癌变过程中肝组织gp96蛋白主要定位于细胞浆,表达水平从变性组到癌前组到癌变组呈明显的梯度增加,均显著高于对照组肝组织(P<0.01)。结论gp96参与肝细胞的癌变过程,癌变初期的过度表达有助于肝癌的早期诊断。

Objective To investigate the character of expression and alteration of heat shock protein （HSP） gp96 during hepatocellular carcinoma（HCC） development. Methods Rat HCC models were induced with 2-acetamidoflurene （2-FAA） in male SD rats. The location of gp96 in rat liver tissues were analyzed by immunohistochemistry and the expression levels were semi-quantitatively detected by Western blot. Results Histological examinations suggested that hepatocytes in rats fed with 2-FAA showed vacuole-like denaturations at the early stages, then dysplastic nodules appeared at the middle stage, and finally progressed to tubercles of cancerous nest. During the process, the liver gp96 protein was mostly located in cytoplasm and an increasing tendency of its levels was found from degeneration to precancerous to cancerous tissues,all of them were significant higher than that in normals （P 〈0.01 ）. Conclusion HSP gp96 involved in HCC development and its overexpression could be a useful marker for early diagnosis of HCC.