5-aza-2′脱氧胞啶联合组蛋白乙酰基转移酶抑制剂LAQ对体外RD-ES细胞的抑制作用

Inhibitory Effects of 5-Aza-2'-Deoxycytidine and Histone Deacetylase Inhibitors LAQ on RD-ES Cells

研究5-杂氮-2'-脱氧胞啶(5aza-CdR)联合组蛋白乙酰基转移酶(HDAC)抑制剂LAQ对体外RD-ES细胞的抑制作用。体外培养人尤文氏瘤细胞株RD-ES,观察不同浓度5aza-CdR联合HDAC抑制剂LAQ对RD-ES细胞的生长抑制作用。本实验表明HDAC抑制剂能增强5aza-CdR对RD-ES细胞抑制作用,且呈量-效关系,LAQ对RD-ES细胞系的IC50值是8 ng/mL,5aza-CdR联合LAQ对体外RD-ES细胞的抑制作用显著,并与瘤抑制基因ECAD和TSLC1的表达密切相关。5-az,a-2'脱氧胞啶联合HDAC抑制剂LAQ对RD-ES细胞生长有明显的抑制作用。

Studied on the inhibitory effects of 5aza-CdR in combination with HDAC inhibitors on vitro RD-ES cells.The HDAC inhibitors LAQ824 was investigated can enhance 5aza-CdR inhibition effect on RD-ES cells by MTT. The combination of 5Aza-CdR and LAQ824 were detected show marked synergy,and be correlated with reactivation for expression of two TSGs：E-cadherin and tumor suppressor lung cancer-1 （TSLC1）. The combination of 5Aza-CdR and Histone Deacetylase Inhibitors LAQ824 were inhibitory effects on RD-ES Cells.