摘要
根据拟南芥ATH1全基因芯片分析毛白杨维管再生过程中基因表达谱的结果,选取了在芯片中特异表达且表达量较高的MYB转录因子家族的7个基因,通过拟南芥突变体研究其对次生维管系统发育的影响及与木材形成相关的关系。通过"三引物法"的纯杂合鉴定和切片显微观察,发现其中1个突变系只有纯合突变体没有杂合体,并且茎基部维管束间纤维细胞壁和野生型相比明显增厚,细胞数量减少,细胞腔也随之减小,推测该突变体可能与次生壁的沉积有关系。
The Affymetrix whole-transcriptome ATH1 chips were used in laboratory to analyze the gene expression profiles during regeneration of secondary vascular system in poplar, and differentially expressed genes were obtained which might involve in cambium initiation, differentiation and xylem development. In this study, the authors chose 7 genes coding for MYB transcription factors that highly expressed during this process, and studied the gene function using Arabidopsis insertion mutants. One of them was found homozygous in mutant alleles and its secondary walls were clearly thickened, while the cell cavity and relatively. Therefore this mutant exhibited changes quantity of interfascicular fiber from inflorescence stem reduced in secondary cell wall deposition.
出处
《林业科学研究》
CSCD
北大核心
2010年第2期170-176,共7页
Forest Research
基金
国家自然科学基金项目(30771697,30671656)
国际先进农业科学技术(948)重大创新项目(2006-4-C01)
关键词
拟南芥
突变体
MYB
木材形成
次生壁
Arabidopsis thaliana
mutation
MYB
wood formation
secondary cell wall
