以糖类利用能力变化选育红霉素高产菌株

Screening of high erythromycin-producing strains by the changes of carbohydrate utilization capacity

目的根据代谢网络理论,利用可能对红霉素产生菌Saccharopolyspora erythraea的代谢物流产生影响的糖类建立理性化的筛选模型。方法建立葡萄糖、果糖、乳糖等抑制性筛选模型和麦芽糖、木糖等利用性筛选模型。将诱变处理后的菌株通过这些模型,找出利用率发生改变的菌株。结果结果显示葡萄糖、麦芽糖模型的筛选效果较好,用此方法筛选出的菌株Er071610-1比原始出发菌株增产53.7%。结论以糖类利用能力变化选育红霉素高产菌株是一条可行的筛选方法。

Abstract Objective According to the theory of metabolic network, set up rational screening models of carbohydrate which may affect Saccharopolyspora erythreaea＇s flux. Methods Utilizing screening models, including inhibitory types（glucose, fructose, lactose, et al） and used types（maltose, xylose, et al）, find out strains which changed carbohydrate utilization rate. Results The results showed that the glucose and maltose model are better than others. The productions of Er071610-1 which screened by this method are 53.7% higher than original strain. Conclusion Changed carbohydrate utilization capacity of strains is a good method to screening high erythromycin-producing strains.