硼替佐米对人类多发性骨髓瘤细胞系U266细胞抗凋亡蛋白HSP-90的影响

Effect of anti-apoptotic protein HSP-90 in human multiple myeloma cell line U266 cells with bortezomib

目的 探讨硼替佐米对人类多发性骨髓瘤（MM）细胞系U266细胞抗凋亡蛋白HSP-90的影响.方法 采用RT-PCR技术研究经不同浓度的硼替佐米作用于U266细胞4 h后其内HSP-90mRNA表达情况的变化.结果 随着硼替佐米浓度的增加,其MM细胞中HSP-90α的mRNA表达量逐渐增加.由低浓度组到高浓度组（0、50、100、150、200 nmol/L）所对应的HSP-90α的灰度值分别为0.343±0.017、0.505±0.039、0.640±0.029、0.760±0.059、0.963±0.054;两两组间比较差异有统计学意义（P〈0.05）.浓度由低到高组对应HSP-90β的灰度值分别为0.610±0.022、0.765±0.050、0.645±0.052、0.770±0.059、0.790±0.027,而HSP-90β的灰度值0 nmol/L组与50、150、200nmol/L组之间差异有统计学意义（P〈0.05）.50、100 nmol/L浓度组对应的HSP-90β的灰度值不同（P〈0.05）.100 nmol/L组与150、200 nmol/L组对应的HSP-90β的灰度值差异有统计学意义（P〈0.05）.HSP-90β的mRNA表达量增加趋势不很明显,但总体差异仍具统计学意义（P〈0.05）.结论 硼替佐米可以上调HSP-90的分子水平的表达,并且以上调HSp-90α的表达为主.

Objective To investigate the effect of anti-apoptotic protein HSP-90 in human multiple myeloma cell line U266 cells after bertezomib interaction. Methods The HSP-90 mRNA expression in the U266 cells was tested by reverse transcription polymerase chain reaction （RT-PCR） after 4 hours of treatment of bertezomib by different concentration. Results With the of increased concentration of bortezomib, the expression level of HSP-90 αmRNA was also increased in U266 cells. Respectively, quantitative results of HSP-90α are 0.343±0.017, 0.505±0.039, 0.640±0.029, 0.760±0.059, 0.963±0.054 from the low to high concentration of bertezomib groups. And there are statistical difference between each group（P 〈0.05）. However, the HSP-90β quantitative results in 0 nmol/L concentration of bertezomib （0.61±0.022） have statistical difference between 50, 150, 200 nmol/L groups（P 〈0.05）. HSP-90β quantitative results in 50（0.765±0.050）and 100 nmol/L（0.645±0.052） nmol/L groups are different（P 〈0.05）. Compared with 100 nmol/L concentration of bortezomib group, statistical difference also exists in 150 （0.770±0.059） and 200 nmol/L （0.790±0.027）groups （P 〈0.05）. Although there is no obvious increase in the mRNA expression of HSP-90β from the chart, statistical difference existed in the whole data （P 〈0.05）. Conclusion Bortezomib can increase the level expression of HSP-90 mRNA, and especially increase the level expression of HSP-90α mRNA.