应用表面增强激光解吸电离飞行时间质谱筛选大肠癌血清特异标志物

Specific Serum Protein of Colorectal Cancer Detected by Surface-enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry

目的采用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测大肠癌患者与健康对照者血清中蛋白的差异,为大肠癌的早期诊断提供理论依据。方法应用SELDI-TOF-MS技术及弱阳离子蛋白质芯片(WCX2)检测36例健康成人(对照组)和36例大肠癌患者(大肠组)血清中蛋白含量。结果在质荷比为2000Da～30000Da的范围内,两组样本检测到了对照组82个蛋白峰。其中3604.240Da蛋白峰大肠癌组明显高于对照组(P<0.05),而12861.368Da的蛋白峰对照组明显高于大肠癌组(P<0.05)。利用这两个蛋白筛查大肠癌的特异度为86.11%(31/36),敏感度为77.78%(28/36),阳性预测值为87.5%。结论质荷比为3604.240Da与12861.368Da的两个蛋白峰可以作为大肠癌早期诊断的备选标志物。

Objective To detect the specific serum protein of colorectal cancer by surface-enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS）and provide the foundation for early detection of colorectal cancer. Methods The levels of serum protein of 36 patients with colorectal cancer and 36 healthy control subjects were detected by weak cation exchanger protein chip（WCX2） chip and SELDI-TOF-MS. Results At the M/Z value range from 2 000 Da to 30 000 Da,82 protein peaks were found. The level of 3 604.240 Da protein was significantly higher in patients with colorectal cancer than in healthy control subjects,while the level of 12 861.368 Da protein was significantly higher in healthy control subjects than in patients with colorectal cancer （P 0.05）. The specificity,sensitivity, and positive predictive value of the detection of colorectal cancer using these 2 protien were 86.11%,77.78%,and 87.5%,respectively. Conclusion The 3 604.240 Da and 12 861.368 Da proteins can be used as the serum marker for early detection of colorectal cancer.