经穴注射骨髓间充质干细胞对后肢缺血大鼠血管活性物质的影响

Influences of meridian point injection of bone marrow mesenchymal stem cells on vaso-active substances in rats with hindlimb ischemia

目的探讨血管活性物质在经穴注射骨髓间充质干细胞(BM-MSCs)改善后肢缺血大鼠血流中的作用。方法SD大鼠24只,随机分为4组:空白对照组、模型组、经穴注射组、肌肉注射组。采用离断股动脉及其分支的方法制造大鼠后肢缺血模型。经穴注射组取三阴交、后三里、照海、环跳、阳陵泉5穴,肌肉注射组取缺血后肢大腿任5个非穴位点,2组均注射BM-MSCs。注射BM-MSCs 3周后,大鼠经腹主动脉取血检测血清中血管紧张素Ⅱ(AngⅡ)和一氧化氮(NO)的含量;取各组大鼠左侧后肢内收肌和腓肠肌,免疫组织化学法测定后肢骨骼肌AngⅡ和内皮素-1(ET-1)的表达量。结果与空白对照组、模型组相比,经穴注射组和肌肉注射组血清内NO的含量显著升高(P<0.01)、AngⅡ含量差别无统计学意义,后肢骨骼肌ET-1表达量显著升高(P<0.01)、而且经穴注射组高于肌肉注射组(P<0.01),后肢骨骼肌AngⅡ含量差别无统计学意义。结论ET-1和NO都参与了经穴注射BM-MSCs后的缺血大鼠后肢血流的改善,AngⅡ未参与经穴注射BM-MSCs后缺血大鼠后肢血流的改善。

Objective To investigate the effects of vasoactive substances and in the relief of rat hindlimb ischemia after meridian point injection of bone marrow mesenchymal stem cells（BM-MSCs）.Methods SD rats（n=24） were randomly divided into the blank control group,model group,meridian point injection group（point group） and muscle injection group（muscle group）.The model of hindlimb ischemia was established in rats by using apocope of femoral artery and branches.The acupoints of Sanyinjiao（SP 6）,Housanli,Zhohai（K 16）,Huantiao（GB 30） and Yanglingquan（GB 34） were chosen in the point group and random 5 points were chosen in the muscle group,and then injected BM-MSCs respectively.After 3 weeks the serum content of angiotensinⅡ（AngⅡ） and nitric oxide （NO） in abdominal aorta were detected,and AngⅡcontent and the expression of endothelin-1（ET-1） in hindlimb skeletal muscle were detected by using immunohistochemistry assay in all groups.Results The serum NO content increased significantly in the point group and muscle group than that in the blank group and model group（P〈0.01）,while the difference in serum content of Angll was not statistically significant.The expression of ET-1 in hindlimb skeletal muscle increased significantly（P〈0.01） in the point group and muscle group,and that in the point group was higher than that in the muscle group（P〈0.01）.The difference of AngⅡcontent in hindlimb skeletal muscle was not statistically significant. Conclusion The results showed that ET-1 and NO joined into the relieve of rat hindlimb ischemia after meridian point injection with BM-MSCs,and AngⅡdid not.