摘要
目的探讨肾炎宁对IgA肾病大鼠肾组织TGF-β_1/p38MAPK信号通路的作用。方法将SD大鼠随机分为4组,对照组、模型组、西药组(贝那普利+氯沙坦)、中药组(肾炎宁),应用脂多糖+牛血清白蛋白+四氯化碳方法复制IgA肾病模型,按各组相应药物剂量灌胃。于第15周末行IgA免疫荧光检测,并用RT-PCR法检测肾组织转化生长因子-β_1(transforming growth factor-β_1,TGF-β_1)、p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinase,p38MAPK)mRNA表达水平。结果中药组、西药组与模型组比较IgA免疫荧光强度、TGF-β_1、p38MAPK mRNA表达水平显著下降(P<0.05)。结论肾炎宁可能通过抑制肾组织TGF-β_1/p38MAPK信号通路,发挥对IgA肾病的治疗作用。
Objective To discuss the influences of Shenyanning on renal signal pathway of transforming growth factor-β1TGF-β1 and p38 mitogen activated protein kinase（p38MAPK） in rats with IgA nephropathy.Methods All SD rats were randomly divided into the control group,model group,Western medicine group（benazepril and losartan）,and Chinese medicine group（Shenyanning）.The model of IgA nephropathy was established by the method of lipopolysaccharide＋bovine albumin＋carbon tetrachloride.All groups were given relevant drugs intragastrically.The expressions of TGF-β1and p38MAPK-mRNA were detected by applying IgA immunofluorescence test and RT-PCR at the end of the 15th week.Results The strength of IgA immunofluorescence and expressions of TGF-β1and p38MAPK-mRNA decreased significantly in the Chinese medicine group and Western medicine group compared with those in the model group（P〈0.05）.Conclusion Shenyanning may cure IgA nephropathy through inhibiting renal signal pathway of p38MAPK.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第3期175-178,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
河北省科技攻关计划项目(No.00276205D)
