鬼箭羽不同提取部位对糖尿病大鼠药理作用的研究

Pharmacological effects of different extract fractions from Guijianyu(Euonymus alatus) on diabetic rats

目的研究鬼箭羽不同提取部位对糖尿病大鼠的药理作用,追踪有效部位,为进一步明确鬼箭羽治疗糖尿病的有效成分和建立其质量控制标准打下基础。方法腹腔注射链脲菌素(STZ)复制大鼠糖尿病模型,并分为模型组、治疗组,健康大鼠为正常组。治疗组分别给予鬼箭羽不同提取部位即石油醚、乙酸乙酯、正丁醇、水、残渣、精制多糖部位。4周后观察体重增加量及血糖、血脂、肌酐、尿素和全血黏度、全血高/低切还原黏度等。结果石油醚、水和乙酸乙酯部位降糖作用明显;正丁醇和精制多糖部位降血肌酐作用明显,其余各组降血尿素作用显著;残渣部位能明显降低低密度脂蛋白、胆固醇含量;水部位降低血液黏度作用明显,精制多糖部位能显著提高红细胞压积;除精制多糖组体重减轻外其余治疗组体重均增加。结论鬼箭羽各提取部位在降糖、降脂、改善肾功能、降低血液黏度、增加体重方面作用各有侧重,单味鬼箭羽可通过影响糖尿病发病的不同环节治疗和预防糖尿病及其并发症。

Objective To study the Pharmacological effects of different extract fractions from Guijianyu （Euonymus alatus） on diabetic rats,pursue the effective fractions,make clear the effective constituents of Guijianyu for treating diabetes,and set up the quality control standard.Methods The model of diabetic rat was established by the injection of streptozotocin（STZ）.All rats were divided into the model group,treatment group and normal group.The treatment group was given the fractions of Guijianyu including petroleum ether, ethyl acetate,n-butyl alcohol,water,residue and rectified polysaccharide respectively.After 4 weeks the body weight,blood sugar（BS）,blood fat,creatinine,urea,and whole blood viscosity（WBV） and whole blood reduced viscosity at high or low share rate were observed.Results The fractions of petroleum ether, water and ethyl acetate had significant anti-diabetes effect,n-butyl alcohol and rectified polysaccharide had significant reducing effect on blood creatinine,and other fractions had significant reducing effect on urea.The fraction of residue decreased significantly the content of low-density lipoprotein（LDL） and cholesterol（CH）, water decreased WBV,and rectified polysaccharide increased significantly hematocrit（HCT）.The body weight was put on by all fractions except rectified polysaccharide.Conclusion Different fractions of Guijianyu have different effects in anti-diabetes,anti-hyperlipidemia,improving kidney function,decreasing blood viscosity and putting on body weight.Guijianyu can be used for curing and preventing diabetes and its complications through influencing different links in the development of diabetes.