2型糖尿病患者血清白介素10水平及其基因启动子592位点多态性与2型糖尿病的关系

Relationship of Serum Interleukin-10 Level and Its Gene Promoter 592 Polymorphism to Type 2 Diabetes Mellitus

目的观察2型糖尿病患者血清IL-10水平及IL-10基因启动子592位点多态性与2型糖尿病易感性的关系。方法选择224例2型糖尿病患者(DM组)和275例健康对照者(NC组)。使用酶联免疫吸附法(ELISA)测定两组受检者血清IL-10水平;并从人体抗凝血白细胞中提取DNA,采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法分析IL-10-592基因多态性。结果DM组血清IL-10水平为(21.1±5.2)pg/ml,对照组为(42.4±6.3)pg/ml,二者间差异有统计学意义(P<0.01)。DM组IL-10-592基因型频率分别为:纯合突变型(AA)36例(16.1%),杂合子(AC)122例(54.5%),野生型(CC)66例(29.4%);NC组:纯合突变型24例(8.7%),杂合子138例(50.2%),野生型113例(41.1%)。DM组和NC组等位基因频率:A分别为43.3%和33.8%,C分别为56.7%和66.2%。两组IL-10-592基因型频率及等位基因频率间差异均有统计学意义(P<0.01)。结论2型糖尿病患者血清IL-10水平显著低于健康者,IL-10可能对糖尿病发病及预后、保健、康复具有保护作用;IL-10基因启动子区592位点多态性可能与2型糖尿病的遗传易感性相关。

Objective To investigate the relationship of serum IL - 10 level and its gene promoter 592 polymorphism to type 2 diabetes mellitus （T2DM） susceptibility. Methods Two hundred twenty -four T2DM patients （DM group） and 275 normal subjects （control group） were enrolled from Tangshan region of Hebei Province. DNA was extracted from white cells in human anticoagulant plasma to analyze IL - 10 - 592 gene polymorphism by polymerase chain reaction - restriction fragment length polymorphism （PCR - RFLP）. Results There was significant difference in serum IL - 10 level between groups DM （21.1 ± 5.2 pg/ml） and control （42.4 ± 6. 3 pg/ml） （ P 〈 0. 01 ）. IL - 10 - 592 genotypic frequencies were 16. 1% （ AA）, 54. 5% （AC）, 29.4% （CC）, respectively, in DM group, and 8.7% （AA）, 50.2% （AC）, 41.1% （CC）, respectively, in control group. The allele frequencies of groups DM and NC were 43.3% , 33.8%, respectively, for A, and 56. 7% , 66. 2% , respectively for C. The difference was significant between 2 groups （P 〈 0. 01 ）. Conclusion The IL - 10 levels are significantly lower in T2DM patients than in healthy subjects. IL - 10 may play a protective role in diabetic incidence, health care and rehabilitation. IL - 10 - 592 polymorphism may be related to T2DM genetic susceptibility.