彩色甜椒基因组DNA提取方法的优化

Optimization of Extraction Methods for Genomic DNA from Color Sweet Pepper

以不同颜色的甜椒为试验材料,采用CTAB法、SDS法、ROSE法和氯化苄法提取彩色甜椒基因组DNA。结果表明,CTAB法所提取DNA的质量和纯度较高,以此DNA为模板进行RAPD-PCR分析,DNA扩增效果较好,带形清晰、整齐,说明CTAB法提取的DNA较为完整,能用作RAPD-PCR模板来开展彩色甜椒分子水平的研究。

Benzyl chloride method, CTAB, SDS, and ROSE methods were used to extract DNA from color sweet pepper leaves of different fruit color. The results of UV-sepectrophotometry, agarose gel elec- trophoresis and PCR showed that, CTAB method was suitable for color sweet pepper. RAPD-PCR with DNA extracted by CTAB as templates showed that its amplification was good, clear and uniform. DNA extracted by CTAB could be used as templates for molecular biological research in color sweet pepper.