DLC薄膜中sp^2键含量对HUVEC细胞附着性能的影响

Influence of sp^2 content on the adhesion behavior of HUVEC

本文利用具有特殊的电子结构的类金刚石(DLC)薄膜来研究材料表面电子结构对人脐静脉内皮细胞(HUVEC)附着性能的影响。采用直流电弧脉冲等离子体沉积法制备DLC薄膜,通过改变靶室气体种类和气体压强来控制DLC薄膜中sp2键的含量,并通过Raman光谱分析法进行测定。采用DAPI免疫荧光染色法对细胞附着形态进行观察,并利用ImageJ对随机记录的多个视场的所有细胞进行了细胞附着形态的统计学分析。实验结果表明:在充入Ar气制备的DLC薄膜表面,内皮细胞的附着性能随着sp2键含量的升高而变差,充入Ar/C2H2混合气体制备的DLC薄膜因所有细胞附着形态均很差而未体现出随sp2键含量而变化的明显趋势。

In this paper, Diamond -Liked Carbon （DLC） Films with different sp2 content were used to investigate the influence of electric property on the adhesion behavior of HUVEC （ human umbilical vein endothelial cells）. The DLC films were prepared by means of Pulsed Arc Plasma Sputtering Deposition. The relative content of sp2 bond of DLC films was adjusted by alternate the species and flow rate of the chamber gas, and was detected by Raman spectroscopy. The adhesion configuration of HUVEC was observed by immunofluorescent staining method, and hnage J software was used to statistically analyzing the configuration of the adhered cells. The results show that ： on the DLC films that are prepared in Ar atmosphere, the adhesion state of HUVEC tencls to be worse as the content of sp2 bond increasing; while on the films that are prepared in Ar/C2H2 atmosphere, the adhesion state of HUVEC is so bad that no significant tendence was found.