Bt杀虫蛋白基因导入新疆棉花获得抗虫转基因植株

The Acquirement of Transgenic Bollworm Resistant Xinjiang Cotton Plants with the Synthetic Gene Coding Bacillus Thuringiensis Insecticidal Protein

通过花粉管通道法将人工全序合成的Ｂｔ杀虫蛋白基因导入新陆早４号和Ｃ６５２４两品种中，收获注射过Ｂｔ杀虫蛋白基因的棉花种子２万余粒。经棉铃虫饲喂的抗虫性生物检测，得到高抗虫的１８株新陆早４号转化苗和３６株Ｃ６５２４转化苗；以Ｂｔ基因的一对引物进行ＰＣＲ分析，５４株抗棉铃虫的转化棉苗均扩增出部分Ｂｔ杀虫蛋白基因的目标带。未转化的对照棉苗则无此条带，证实Ｂｔ杀虫蛋白基因已整合到转化抗虫棉株的染色体上，首次获得了新疆转目的基因的高效抗虫棉。

By the pollon tube pathway, the artificially synthesized Bacillus thuringiensis insecticidal protein gene was transformed into the elite Xinjiang cotton( Gossypium hirsutum L.) cultivars namely Xinluzao No.4 and C6524, and 20 thousand injected cotton seeds were obtained. After the biological inspection by raised bollworm ( Helicoverpa armigera ), 18 Xinluzao No.4 plants and 36 C6524 plants resisting to bollworms were got. With a pair of primers of Bt gene, the results of PCR analysis indicated that the Bt gene did integrate within the genenome of the total 54 transformed bollworm resistant cotton plants. We primarily got the transgenic bollworm resistant Xinjiang cotton plant.