摘要
通过花粉管通道法将人工全序合成的Bt杀虫蛋白基因导入新陆早4号和C6524两品种中,收获注射过Bt杀虫蛋白基因的棉花种子2万余粒。经棉铃虫饲喂的抗虫性生物检测,得到高抗虫的18株新陆早4号转化苗和36株C6524转化苗;以Bt基因的一对引物进行PCR分析,54株抗棉铃虫的转化棉苗均扩增出部分Bt杀虫蛋白基因的目标带。未转化的对照棉苗则无此条带,证实Bt杀虫蛋白基因已整合到转化抗虫棉株的染色体上,首次获得了新疆转目的基因的高效抗虫棉。
By the pollon tube pathway, the artificially synthesized Bacillus thuringiensis insecticidal protein gene was transformed into the elite Xinjiang cotton( Gossypium hirsutum L.) cultivars namely Xinluzao No.4 and C6524, and 20 thousand injected cotton seeds were obtained. After the biological inspection by raised bollworm ( Helicoverpa armigera ), 18 Xinluzao No.4 plants and 36 C6524 plants resisting to bollworms were got. With a pair of primers of Bt gene, the results of PCR analysis indicated that the Bt gene did integrate within the genenome of the total 54 transformed bollworm resistant cotton plants. We primarily got the transgenic bollworm resistant Xinjiang cotton plant.
出处
《西北农业学报》
CAS
CSCD
1998年第4期1-3,共3页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家高技术研究与发展计划
国家九五重大科技攻关资助
关键词
棉花
Bt杀虫蛋白基因
棉铃虫
花粉管通道法
Cotton
Bollworm
Pollon tube pathway
Bacillus thuringiensis insecticidal protein gene