摘要
RNA提取技术是现代分子生物学研究中一项重要的常用实验技术。本文简要介绍了一种从动物组织中提取高纯度、高质量总RNA的方法。该方法是在Bioteke试剂盒基础上进行优化和改进,具有实用性强、重复性好的特点。通过紫外分光光度仪和琼脂糖凝胶电泳检测所提取的RNA,结果表明:通过优化后提取的总RNA的完整性和纯度较高,完全能满足分子克隆和基因表达研究的需要。
The technique of extracting total RNA is frequently used in modem molecular biology research. A modified method by which obtained high purity and yield of total RNA purification from animal tissue was introduced in this paper. This method which was modified based on the bioteke kit has practical and repeatable features. By using ultraviolet absorption spectroscopy and agarose gel electrophoresis to test RNA. The results indicated that with these modifications, the pure and integral RNA could be obtained, which was suitable for molecular cloning and genes expression researches.
出处
《湖南农机（学术版）》
2010年第1期78-79,82,共3页
Hunnan Agricultural Machinery
基金
云南省教育厅基金项目(500937)资助
关键词
RNA提取
性腺组织
RNAPurification
Gonadal tissue
