期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

基于甲基化DNA分离方法的甲基化DNA检测和全基因水平的甲基化分析 被引量：1

Methylated DNA Isolation Assay-Mediated DNA Methylation Detection and Whole-Genome Methylation Profiling
下载PDF
导出
摘要 已知异常的DNA甲基化对基因表达有明显影响并且涉及包括癌症等的疾病以及正常状态,因此从非甲基化DNA中鉴定甲基化DNA的分析技术是非常必须的。本文介绍了一种利用甲基化DNA结合域对甲基化DNA的亲和性质将甲基化DNA从全基因中成功提取的甲基化DNA分离技术(MeDIA)。结合MeDIA的PCR和CpG芯片技术分别实现了局部位置甲基化DNA状态的评估以及全基因的甲基化分析。 The ability to distinguish methylated DNA from nonmethylated DNA is essential because aberrant DNA methylation is known to have significant effects on gene expression and is involved with normal development as well as diseases,including cancer.This article describes a method of methylated DNA isolation assay（MeDIA） that is designed for the successful extraction of methylated DNA from genomic DNA of interest by utilizing the affinity property of methyl DNA binding domain to methylated DNA.PCR and CpG microarrays coupled with MeDIA are able to assess a locus-specific methylation status and genome-wide methylation profile,respectively.
作者 Young Ho Moon TaeJeong Oh Na Young Kim Myung Soon Kim Sungwhan An
出处 《生命科学仪器》 2010年第1期54-57,共4页 Life Science Instruments
关键词 甲基化分析 DNA检测 基因水平 分离方法 DNA甲基化 DNA结合域 分离技术 MeDIA
分类号 Q523 [生物学—生物化学]
  • 相关文献

参考文献16

  • 1Herman J G,Baylin S B.Mechanisms of disease:gene silencing in cancer in association with promoter hypermethylation.N Eng J Med,2003,349:2042-54.
  • 2Singer-Sam J,Grant M,LeBon J M,Okuyama K,Chapman V,Monk M,Riggs A D.Use of a Hpall-polymera.chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.Mol Cell Biol,1990,10:4987-9.
  • 3Herman JG,Graff J R,Myohanen S,Nelkin B D,Baylin S B.Methylationspecific PCR:a novel PCR assay for methylation sta tus of CpG islands.Proc Natl Acad Sci,USA 1996,93:9821-6.
  • 4Gebhard C,Schwarzfischer L,Pham T H,Andreesen R,Mackeasen A,Rehli M.Rapid and sensitive detection of CpGmethylation using methyl-binding(MB)-PCR.Nucl Acids Res,2006,34:e82.
  • 5Suzuki H,Gabrielson E,Chen W,Anbazhagan R,Van Engeland M,Weijenberg M P,Herman J G,Baylin S B.A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancer.Nat Genet,2002,31:141-9.
  • 6Brena R M,Huang T H,Plass C.Quantitative assessment of DNA methylation:potential applications for disease diagnosis,classification,and prognosis in clinical settings.J Mol Med,2006,84:365-77.
  • 7Callinan P A,Feinberg A P.The emergirig science of epigenomics.Hum Mol Gene,.2006,15:R95-R101.
  • 8Fohz G,Ryu G Y,Yoon J G,Nelson T,Fahey J,Frakes A,Lee H,Field L,Zander K,Sibenaller Z,Ryken T C,Vibhakar R,Hood L,Madan A.Genome-wide analysis of epigenetie silencing iden titles bex1 and bex2 as candidate tumor suppressor genes in malignant glioma.Cancer Res,2006,66:6665-74.
  • 9Khulan B,Thompson R F,Ye K,Fazzari M J,Suzuki M,Stasiek E,Figneroa M E,Glass J L,Chen Q,Montagna C,Hatchwell E,Selzer R R,Richmond T A,Green R D,Melnick A,Greally J M.Comparative isoschizomer profiling of cytosine methylation:the HELP assay.Genome Res,2006,16:1046-55.
  • 10Schumacher A,Kapranov P,Kamimky Z,Flanagan J,Assadzadeh A,Yau P,Virtanen C,Winegarden N,Cheng J,Gingcras T,Petronis A.Microarray-based DNA methylation profiling:technol ogy and applications.Nucl Acids Res,2006,34:528-42.

同被引文献9

引证文献1

二级引证文献1

生命科学仪器
2010年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部