EB病毒BZLF1N基因的克隆与序列分析

Molecular cloning and sequence analysis of Epstein-Barr virus BZLF1N gene

目的:克隆EB病毒BZLF1N基因编码区的cDNA并对其序列进行分析。方法:采用RT-PCR方法,从B95-8细胞获得BZLF1N基因的cDNA,克隆至pGEM-TEasy载体,选择阳性克隆并进行序列测定。结果:构建的重组载体中含有EB病毒BZLF1N基因的全长序列,与Genebank公布的序列完全一致。结论:获得EB病毒BZLF1N基因的克隆,为进一步的研究奠定了基础。

Objective To clone and analyze the full-length cDNA encoding Epstein-Barr virus （EBV） BZLF1N gene. Methods The eDNA of EBV BZLF1N gene was amplified by RT-PCR using the total RNA extracted from B95-8 cell.The PCR product was cloned into pGEM-T Easy vector and then transferred into E.coli DH5α.The positive recombinant clone was analyzed by restriction endonuclease digestion and DNA sequencing. Results The recombinant plasmid had a complete open reading frame of EBV BZLFIN and shared 100% homology with the sequence of EBV BZLF1N in Genebank. Conclusions The cDNA of Epstein-Barr virus BZLF1N gene is cloned successfully,which will lay the foundation for further research.