LOX-1基因501G>C和IVS4-73C>T多态性与湖南汉族人不稳定型心绞痛发病的相关性

Association between 501G>C and IVS4-73C>T polymorphisms of the oxidized low density lipoprotein receptor-1 (LOX-1) gene and pathogenesis of unstable angina pectoris in Hunan Han population

目的:研究血凝素样氧化性低密度脂蛋白受体-1(LOX-1)基因501G>C和IVS4-73C>T多态性与不稳定型心绞痛(UAP)发病的相关性。方法:运用PCR-RFLP检测LOX-1基因501G>C和IVS4-73C>T单核苷酸多态性(SNP)位点基因型,观察其多态性的基因型及等位基因在150例湖南籍汉族人UAP患者和146例健康对照中的分布频率。结果:病例组和对照组的LOX-1501G>C基因型和等位基因频率分布差异无统计学意义(P=0.248和P=0.109),含有突变基因的(GC+CC)基因型与野生型GG基因型的频率分布差异无统计学意义(OR=0.671,P=0.131);LOX-1IVS4-73C>T基因型和等位基因频率分布差异无统计学意义(P=0.756和P=0.559),含有突变基因的(CT+TT)基因型与野生型CC基因型的频率分布差异亦无统计学意义(OR=0.886,P=0.692)。结论:湖南汉族人群中LOX-1501G>C和IVS4-73C>T多态性与UAP发病无明显相关性。

Objective To investigate the association between 501G 〉 C and IVS4-73C 〉 T polymorphisms of the lectin-like oxidized low density lipoprotein receptor-1 （LOX-1 ） gene and pathogenesis of unstable angina pectoris （UAP） in HuMan HaM population. Methods The genotypes and alleles of 501G 〉 C and IVS4-73C 〉 T single nucleotide polymorphisms （SNP）of LOX-1 gene were detected by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） method in 150 cases with UAP （patient group） and 146 normal controls （control group） of HuMan HaM population. Results No obvious difference of frequency distribution of genotypes and alleles in 501G 〉 C （P = 0.248, P = 0.109） or IVS4-73C 〉 T （P = 0.756, P = 0.559）, and of frequency distribution of 501C allele carriers （CG＋ CC genotypes）（OR = 0.671,P = 0.131） or IVS4-73T allele can＇iers （CT＋TF genotypes） （OR = 0.886,P = 0.692） was observed between the two groups. Conclusion It is suggested that LOX-1 501G 〉 C and IVS4-73C 〉 T SNPs are not obviously associated with pathogenesis of UAP in HuMan HaM population.