p,p'-DDD对人乳腺癌细胞的增殖作用

Study of the Proliferation Effect of DDD on Mammary Cancer Cell MCF-7

[目的]观察对,对-二氯二苯基二氯乙烷(p,p'-DDD)对人乳腺癌(MCF-7)细胞增殖的影响。[方法]采用体外培养MCF-7细胞增殖试验检测DDD的类雌激素活性,并用生长曲线对其作用机制进行初步探讨。[结果]DDD染毒剂量在3×10-7mol/L、3×10-6mol/L时,存在刺激MCF-7细胞增殖的类雌激素活性(P<0.05),在3×10-6mol/L剂量时其增殖效应最大。细胞生长曲线方面,DDD组在第3、5、7天各时间段细胞数均明显高于溶剂对照组(P<0.05)。[结论]DDD在3×10-7mol/L、3×10-6mol/L具有刺激MCF-7细胞增殖的类雌激素活性。

[ Objective ] To study the proliferation effect of p,p＇-DDD on human mammary cancer cell MCF-7. [ Methods ] We detected estrogenic activity of DDD using the MCF-7 cell proliferation experiment in vitro, and analysed its mechanism by means of growth curve. [ Results ] DDD in the dose of 3 ×10^-7 mol/L and 3 ×10^-6mol/L could stimulate the MCF-7 cell proliferation and produce estrogenic activity, and the most proliferation effect dose was 3 ×10^-6 mol/L. Celt cycle analysis of cell proliferation index indicated that DDD group was significantly higher than the control group at the third, fifth, and seventh day. [ Conclusion ] DDD in the dose of 3 ×10^-7mol/L and 3 ×10^-6mol/L could stimulate the MCF-7 cell multiplication.