产长链二元酸热带假丝酵母酸分泌过程研究

STUDIES ON SECRETION PROCESS OF Candida tropicalis PRODUCING LONG CHAIN DICARBOXYLIC ACID

热带假丝酵母（C．tropicalis）是长链二元酸发酵生产中常用菌种。其二元酸分泌过程是烧烃代谢过程中的重要步骤，pH在7．4～8．2范围内，足够高的pH对分泌和产酸是必需的。二元酸钠盐明显不利于分泌过程。二元酸的分泌相对于胞内ω一氧化过程是快速过程。建立了一种用于研究分泌过程的方法，利用静息细胞在缓冲溶液中分泌所引起的溶液pH变化来获得酸分泌量的在线数据。

Candida tropicalis is widely used strain in preducing long chain dicarboxylic acid by fermentation. The sccretion of the acid is an imPOrtant step in the metabolic process of hydrocarben. High pH range between 7. 4 - 8. 2 is essential for both the secretion and the production of the acid. anium salt of the dicarboxylic acid is obviously harmuLl to the secretion process. The step of the secretion is a fast process as compared with the co oxidation process within the cell. A methed was established to study the secretion process, i. e. by means of on - line data of dicarboxylic acid obtained by measuring the pH changes caused by the amount of the acid secretion by the resting cells in a buffer.