北京地区人群诺瓦克样病毒血清抗体水平调查

IDENTIFICATION OF SERUM ANTIBODIES AGAINST NORWALK GROUP VIRUSES IN BEIJING POPULATION

为了解诺瓦克样病毒在我国人群中的流行情况，采用间接酶联免疫吸附法（ＥＬＩＳＡ），分别以重组杆状病毒表达的Ｎｏｒｗａｌｋ（ｒＮＶ）和Ｍｅｘｉｃｏ（ｒＭＸ）病毒样颗粒为抗原，检测了北京地区１１０９份不同年龄人群血清标本中的特异性ＩｇＧ抗体。总检出率为ｒＮＶ８８．８％，ｒＭＸ９０．６％。两种型别抗原的抗体分布形式类似：７－１１个月的阳性率最低，分别为ＮＶ４１．４％，ＭＸ３６．２％；１岁时达到ＮＶ６５．２％，ＭＸ６９．６％；３岁时达到ＮＶ８４．６％，ＭＸ８９．７％；８、９岁后均接近１００％。结果提示。

A total of 1,109 serum specimens collected in Beijing were tested by an indirect enzyme-linked immunosorbent assay (ELSA) for specific IgG respectively to Norwalk virus (rNV) and Mexican virus (rMX) using baculovirus-expressed recombinant capsid proteins. Out of 1,109 specimens tested, 89.0% were antibody-positive to NV and 90.6% to MX. The prevalence patterns of antibodies against these two viruses were similar: Antibody prevalence was lowest in 7 to 11 month old infants with 41.4% positive for NV and 36.2% for MX; and increased in 1 year old children to 65.2% for NV and 69.6% for MX; in 3 year old children, it reached 84.6% for NV and 89.7% for MX; and in group aged over 8 or 9 years, it was nearly 100% for both. The data indicate that infections of these two serotypes of Norwalk group viruses in Beijing are very common.