摘要
试验采用顶空固相微萃取(HS-SPME)和气相色谱-质谱联用法(GC-MS)测定了东湖水体的腥味成分和东湖鲤鱼体内挥发性成分,并测定了鲤鱼体内脂肪氧化酶与不饱和脂肪酸花生四烯酸的反应产物。结果表明,东湖水体中土臭素和2-甲基异冰片浓度范围分别为1.67～5.37 ng/L和15.26～29.68ng/L。东湖鲤鱼体内含有腥味物质2-MIB,在不同组织中的浓度范围为1.8～5.1 ng/kg,腥味物质GSM未检测到。鲤鱼体内脂肪氧化酶与底物花生四烯酸反应产生的物质主要有己醛、1-辛烯-3-醇、2-辛烯醛、2-壬烯醛、2,4-癸二烯醛等,主要的反应产物和鲤鱼体内主要的挥发性成分基本吻合。本试验通过产生的主要反应产物的量来间接测定酶的活性,其中酶活最强的是鱼鳞酶,其他依次为鱼鳃酶、鱼血酶、鱼肉酶和内脏酶。
In this paper,the volatile components in carp lyrics and the off-flavor components in East Lake were determined using headspace solid phase microextraction（HS-SPME） coupled with gas chromatography mass spectrometry（GC-MS）,and the main reaction products of arachidonic acid and lipoxidase were also analyzed by GC-MS.The results showed that GSM and 2-MIB in East Lake were 1.67~5.37 ng/L and 15.26~29.68 ng/L respectively.The concentrations of 2-MIB in different tissues in carp lyrics ranged from 1.8 to 5.1 ng / kg,but GSM was not detected.The main reaction products of arachidonic acid and lipoxidase included hexanal,1-octen-ol,2-octenal,2-nonenal and 2,4-decadienal which were similar with those volatile components in carp lyrics.The results revealed that the highest activity enzyme which quantified indirectly by the contents of the main reaction products was from scale,and the decrease enzyme activity order were from gills,blood,muscle and visceral.
出处
《湖北农业科学》
北大核心
2010年第3期688-691,共4页
Hubei Agricultural Sciences
基金
国家科技部国际重点合作项目(2007DFA31220)
