蛛网菌属中分解钾的菌株

A POTASSIUM DECOMPOSE STRAIN IN GENUS ARACHINA

从我国四川省汉源县富泉矿马桑树根际分离到一株能分解钾的菌株，该菌细胞为杆状，近球状。有时具有分支状菌丝，成对V形排列。基丝有分隔，断裂成杆状或球状小体，革兰氏阳性，不运动，兼性厌氧。菌落由初级分支菌丝体组成，灰白色，无气生菌丝，细胞壁含有二氨基庚二酸LL-DAP（左旋），甘氨酸、天门冬氨酸、半乳糖、葡萄糖，无特征性糖；细胞壁组分属Ⅰ型。发酵葡萄糖产生乙酸和异成酸。用原子吸收法测定可溶性钾，比对照增加49．5％，证明能分解钾。DNA中G＋C为63mol％。根据这些性状可归入蛛网菌属（Arachina），但又由于不产丙酸和分解钾故不同于该属唯一的丙酸蛛网菌（Arachinapropionica），因此，可能是一个新种，但由于缺少模式种，尚待进一步研究。另对该菌制剂进行了玉米、水稻的盆栽实验，结果说明经3号菌分解后的生物矿肥的速效钾提高6倍多。因此认为该菌株具有农业应用前景。

A strain of potassium decompose, The culture No. 3, was isolated fromrhizosphere soil of higher plant Coriaria nepalensis at Fu Quano Mine in Han Yuan county, Sichuan province, China. The cell of the strain No. 3 are short irregular rods, coccoid, 0.4-0.6×2.5- 5.0μm some cells branched and some with branchingfilaments. Commonly break up in older cultures into short rods or coccoid. Often with clubbed end, arranged in pairs, in V configuration. Substrat tilament with septa Gram positive, nonmotile, not acid-fast. Facultative anaerobic. Colony are composed of elementary branching filaments; white, and opaque; no aerial filaments. Cell wall contained LL-diaminopimelic acid (LL-DAP), glycine, aspartic acid, glucose and galactose. The major products of glucose fermentation are acetic acid and iso-valeric acid. Atom spectrum absorbtion fermentation determination showed after K decompose treatment the soluble K is 49.5% higher than the control sample so its K decompose effect is evident. G+G ratio is 63mol%. Accordingly the strain No. 3 can be put into genus Arachnia. But is cannot producing propionic acid and with K decompose capacity so it cannot classified as Arachnia propionica. Therefore, it may be a new species of Arachia. However, we cannot definitely say so far that it is a new species of the absence of type spedes. Further investigation is needed in order to make sure it is a new species. In corn and rice pot experiments the K decompose strain No. 3 increasing yield 6 times than the untreated original instant potassium fertilizer added pots. So it has good prospect in future agricultural application.