来自海洋的链霉菌抑菌活性与其培养条件的关系

RELATIONSHIP BETWEEN ANTIMICROBIAL ACTIVITY OF MARINE STREPTOMYCES SPP. AND THEIR CULTURE CONDITIONS

研究培养条件对来自海洋的12株链霉菌抑菌活性的影响。结果表明，用海水（含3．5％NaCl）制备的高氏1号培养基（pH72）、22℃培养时，能表现抑菌活性的菌株数最多；但减少培养基的营养成分、改变氮源、NaCl浓度、pH和培养温度，部分菌株可表现新的抑菌活性，或使原有的抑菌活性有较大提高；新表现或提高的抑菌活性绝大部分都是针对革兰氏阳性菌和白色假丝酵母。本文结果提示在海洋放线菌抗生素产生菌的筛选中，应注意培养条件的选择。

The purpose of this work was to study the effects of the culture conditions on antimicrobial activity of tWelve strains of the marine Streptomyces spp The results showed that the maximum numbers of the marine Streptomyces spp. strains having an antimicrobial activity were obtained when cultured with the starch-nitrate potassium medium (pH7.2) prepared with seawater anp at 22℃. The new or increasing antimicrobial activities of the marine Streptomyces spp. were observed when cultured with diluted the starch-nitrate potassium medium or the medium with different nitrogen source, NaCl concentration, pH or culture temperature. The most new or increasing antimicrobial activities of the marine Streptomyces spp. observed were against Gram-positive bacteria and Candida albicans tested. The results of this work indicate that it is important to choose the culture conditions in screening the antibiotic-producers from the marine actinomycetes.