摘要
以野生型自养黄杆菌为出发菌株,经亚硝酸、紫外线、60Coγ射线诱变处理选得三株性状优良的突变株(Uγ-、Uγ-17、Uγ-20)。Uγ-1突变株的菌落形态为菊花形,白色。Uγ-17和Uγ-20突变株的菌落形态均为圆凸光滑型,微黄色。40h的摇瓶发酵结果表明,Uγ-1、Uγ-17、Uγ-20突变株的干细胞产量分别为8.1g/1、11.2g/1、10.5g/1,其产物对干细胞的得率系数(yp/x)分别为0.74、0.75、0.79。与野生型菌株相比,这些突变株的干细胞产量yp/x分别增加了20%和7.2%以上。
The wild strain of X autotrophicus was mutated by induction of nitrous acid, ultraviolet ray and 60Co γ~ray Three mutant strains(Uγ-1, Uγ-17 and Uγ-20) were obtained by the initial and duplicate selections. The colony morphology of Uγ-1 mutant is chrysanthemum type and it is White. The colony morphology of Uγ-17 and Uγ-20 are circular, smooth and yellowish. The shake flask fermentation was cAned out for 40h. The results showed that the dry cell yield of Uγ-1, Uγ-17 and Uγ-20 mutant strains were 8.1g·L-1, 11.2g·L-1 and 10.5g·L-1 respectively. And the yield of P(HB-co-HV) tO the dry cell (yp/x) of the three mutant strains were 0.74. 0.75 and 0.79 respectively. Compared with the wild strain, the dry cell yield and yp/x of the mutant stains increased by over 20% and 7.2%, respectively.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第6期329-331,共3页
Microbiology China