降钙素基因相关肽基因直接注射大鼠

Direct Injection of Calcitonin Gene Related Peptide Gene in Rats.

所设计的基因表达一个含降钙素基因相关肽（ＣＧＲＰ，３７个氨基酸）、及其Ｎ端信号肽和Ｃ端酰胺化位点的多肽．该基因克隆到哺乳动物表达载体ｐＢＰＶ上并构成ｐＢＰＶＣＧＲＰ．将此质粒ＤＮＡ直接注射自发高血压大鼠并导致其血压在注射样品后３～２２ｄ内明显下降，下降幅度超过了１２％，在第１６天甚至达到了２４４２％．基因直接注射比ＣＧＲＰ本身的降压作用维持时间更久．

The whole designed gene expressing a peptide consisting of calcitonin gene related peptide (CGRP) (37aa) itself, a signal peptide at its N terminus and an amidated site at its C terminus is cloned into mammalian expression vector pBPV. The pBPV CGRP plasmid DNA is directly injected into the spontaneous hypertension rats (SHR) and results in the significant decrease of SHRs blood pressure during 3～22 days after injection. The percent of decrease reaches more than 12%, even up to the top 24 42% on the 16th day. The effect can last for longer time and also be better than that of CGRP protein.