核转录因子结合活性与血管平滑肌细胞增殖的关系

Nuclear Transcription Factor Binding Activity and Proliferation of Vascular Smooth Muscle Cell

通过培养血管平滑肌细胞（ＶＳＭＣ）的直接细胞计数显示自发性高血压大鼠ＳＨＲ的细胞增殖快于正常血压大鼠ＷＫＹ；应用ＤＮＡ－蛋白质凝胶漂移泳动技术比较ＳＨＲ和ＷＫＹ的ＶＳＭＣ核转录因子Ａｐ１、Ａｐ２、ＧＲＥ、ＣＲＥＢ、Ｓｐ１、ＣＴＦ／ＮＦ１、ＮＦ－κＢ结合活性，发现ＳＨＲ的ＣＴＦ／ＮＦ１和ＮＦ－κＢ结合活性高于ＷＫＹ，用Ｐ６５和Ｐ５０抗体证明了ＮＦ－κＢ结合的特异性；同时，ＬＰＳ诱导ＶＳＭＣ和ＰＤＴＣ抑制ＬＰＳ诱导的实验表明ＮＦ－κＢ结合活性增强是通过ＩκＢ磷酸化而激活。本研究提示核转录因子ＮＦ－κＢ对高血压ＶＳＭＣ异常增殖有重要作用。

Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibited exaggerated growth as compared with the cells from normotensive Wistar-Kyoto (WKY) by direct cell count in culture A comparison of nuclear transcription factors Ap1、Ap2、GRE、CREB、Sp1、CTF/NF1、NF-κB binding activity of VSMC between SHR and WKY showed higher CTF/NF1 and NF-κB activity in SHR than WKY P65 Ab and P50 Ab were used to test the specificity of NF-κB binding Moreover, LPS induced NF-κB binding of VSMC, and PDTC suppressed LPS-induced NF-κB DNA-binding So NF-κB may be activated through phosphorylation of IκB These results suggested nuclear transcription factor NF-κB may contribute to the pathogenesis of proliferation of VSMC in hypertension