摘要
通过培养血管平滑肌细胞(VSMC)的直接细胞计数显示自发性高血压大鼠SHR的细胞增殖快于正常血压大鼠WKY;应用DNA-蛋白质凝胶漂移泳动技术比较SHR和WKY的VSMC核转录因子Ap1、Ap2、GRE、CREB、Sp1、CTF/NF1、NF-κB结合活性,发现SHR的CTF/NF1和NF-κB结合活性高于WKY,用P65和P50抗体证明了NF-κB结合的特异性;同时,LPS诱导VSMC和PDTC抑制LPS诱导的实验表明NF-κB结合活性增强是通过IκB磷酸化而激活。本研究提示核转录因子NF-κB对高血压VSMC异常增殖有重要作用。
Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibited exaggerated growth as compared with the cells from normotensive Wistar-Kyoto (WKY) by direct cell count in culture A comparison of nuclear transcription factors Ap1、Ap2、GRE、CREB、Sp1、CTF/NF1、NF-κB binding activity of VSMC between SHR and WKY showed higher CTF/NF1 and NF-κB activity in SHR than WKY P65 Ab and P50 Ab were used to test the specificity of NF-κB binding Moreover, LPS induced NF-κB binding of VSMC, and PDTC suppressed LPS-induced NF-κB DNA-binding So NF-κB may be activated through phosphorylation of IκB These results suggested nuclear transcription factor NF-κB may contribute to the pathogenesis of proliferation of VSMC in hypertension
出处
《遗传》
CAS
CSCD
北大核心
1998年第6期22-25,共4页
Hereditas(Beijing)
基金
国家自然科学基金