新孢子虫速殖子灭活苗对沙鼠的免疫保护性试验

Neospora tachyzoites inactivated vaccine on gerbils rotective immunity test

为验证2种新孢子虫速殖子灭活苗的免疫保护作用,以沙鼠为试验动物,用2种不同佐剂疫苗对沙鼠进行免疫,21 d后用新孢子虫速殖子(1×106/只)进行皮下攻毒,观察死亡情况.试验结果表明,第Ⅰ种疫苗保护率为85%(17/20),第Ⅱ种疫苗保护率为80%(16/20),并在死亡沙鼠组织DNA均扩增出新孢子虫特异的基因片段.

In order to validate the immunological protection effect of two different inactivated vaccines a-gainst Neosporiasis tachyzoites. The gerbils were vaccinated twice with two different adjuvant vaccine. 21 days later with tachyzoites （1 ×10^6 Per gerbils） subcutaneous infection and observation gerbils information for death. The results showed that the protection rate is 85M （17/20）of the first kind vaccine,the other （Ⅱ）vaccine protection rate 80M （16/20）. Amplification Neosporiasis specif fragment from the death gerbils tissue DNA.