复配型苜蓿银纹夜蛾核型多角体病毒制剂对两种夜蛾的毒力测定

Bioassay of the Multiple Autographa californica Nucleopolyhedrovirus (AcMNPV) Pesticides

对防治蔬菜甜菜夜蛾（ＳｐｏｄｏｐｔｅｒａｅｘｉｇｕａＨüｂｎｅｒ）和斜纹夜蛾（ＰｒｏｄｅｎｉａｌｉｔｕｒａＦａｂｒｉｃｉｕｓ）为主并兼治菜螟、菜青虫、小菜蛾等鳞翅目害虫的单一型和复配型苜蓿银纹夜蛾核型多角体病毒（ＡｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ，ＡｃＭＮＰＶ）生物杀虫剂进行筛选。毒力测定结果表明：选用单剂的ＡｃＭＮＰＶＡ株（从甜菜夜蛾虫体增殖获得）的毒力（ＬＤ５０＝１３１．８ＰＩＢ／头）比ＡｃＭＮＰＶＢ株（从草地贪夜蛾细胞株Ｓｆ９中获得）的毒力（ＬＤ５０＝１９４９．４ＰＩＢ／头）高１４．７９倍。ＡｃＭＮＰＶＡ株加苏云金杆菌（Ｂｔ）加斜纹夜蛾核型多角体病毒（ＰｌＮＰＶ）形成的复配型ＡｃＭＮＰＶＡ株制剂对甜菜夜蛾二龄幼虫的ＬＤ５０值（２１．８２～２１．４ＰＩＢ／头）与单剂ＡｃＭＮＰＶＡ株的ＬＤ５０值（１６８．８４～２０４．３６ＰＩＢ／头）比值为９．５～７．７倍。同样复配型ＡｃＭＮＰＶＡ株制剂对斜纹夜蛾二龄幼虫ＬＤ５０（３．１６～１５．４６ＰＩＢ／头）与单剂ＰｌＮＰＶＬＤ５０值（１６．０２～５３．５３ＰＩＢ／头）比值为５．１～３．５倍。说明以Ｂｔ和非耙标害?

The multiple with other biological agent Autographa californica nucleopolyhedrovirus (AcMNPV) pesticide, which is widely used to control Spodoptera exigua and Prodenia litura as well as Artogeia rapat, Hellula untalis, Plutella xylostella , was selected. Tested with 2nd instar S. exigua larvae, the virulence of AcMNPV A strain amplified in S.exigua larvae is higher than that of AcMNPV B strain in Sf9 cells. LD 50 of AcMNPV B (1949.4 PIB/Larva) is 14.79 times more than AcMNPV A (131.8 PIB/Larva).The ratio of LD 50 between AcMNPV A (LD 50 =168.84-240.36 PIB/Larva) and AcMNPV A+Bt (LD 50 =1.4329-128.91 PIB/Larva) is 118-1.5 while the ratio between AcMNPV A and AcMNPV A+Bt+PlNPV (LD 50 = 21.82 -21.4 PIB/Larva) is 7.7-9.5. Tested with 2nd instar P.litura larvae, the ratio of LD 50 between PlNPV (LD 50 =16.02-53.53 PIB/Larva)and PlNP V+Bt(LD 50 =2.9-7.76 PIB/Larva ) is 5.5-6.9 while the ratio between PlNPV and PlNPV+Bt+AcMNPV A (LD 50 =3.16-15.469PIB/Larva) is 3.5-5.1. It shows that the virulence of the multiple AcMNPV A significantly increases. The multiple pesticide of AcMNPV A+Bt+PlNPV named SNB 3 has been successfully used in controlling 5 major leaf pests in vegetable.