玉米胚芽鞘向光性运动的一些特性

SOME CHARACTERISTICS OF PHOTOTROPISM IN MAIZE COLEOPTILES

利用云母片分隔、HPLC分析等方法研究了玉米胚芽鞘向光性运动的特性．云母片阻隔生长素的移动后并不能阻止胚芽鞘的向光性弯曲．若单侧伤害胚芽鞘则能引起类似于向光性的弯曲生长．单侧施加伤害分泌物质亦能使胚芽鞘产生弯曲．HPLC分析表明，蓝光诱导和机械伤害后产生的物质主要是6-methoxy-2-benzoxazolinone.

The characteristics of phototropism in maize coleoptiles were studied by insertinga ndca piece into coleoptile and using HPLC analysis. The results showed that ndca piececould not stop phototropic movement of maize coleoptiles, though it could prevent thetranslocation of auxin. The coleoptile could bend to grow sindlarly to phototropism whenits one side was injured by a razor blade. Bending growth of the coleoptiles was alsofound when a piece of agar containing injurant which was extracted from injuredcoleoptile, was placed on unilateral side of coleoptile with tip excised HPLC analysisindicated that the inhibiting substance produced during the induction by blue light or bymechanical iaiury was 6methoxy-2-benzoxazolinone (MB).