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自身免疫性肝炎肺组织中IL-17A及24p3表达的研究

Expression of IL-17A and 24p3 in lung of autoimmune hepatitis mouse
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摘要 目的探讨自身免疫性肝炎模型肺组织中IL-17A和24p3的表达及其变化,为临床治疗自身免疫性肝炎提供新的实验依据。方法选用刀豆蛋白A(ConA)诱导AIH模型,通过RT-PCR检测不同时间点IL-17A及24p3mRNA在小鼠肺组织中的表达,HE组织染色检查肺组织淋巴细胞浸润程度。结果IL-17A mRNA在AIH小鼠肺组织中有不同程度的表达,在相同时间点24p3 mRNA的表达高于IL-17A,并在12h出现最高水平表达,肺脏在8h出现炎症,72h炎症情况最为严重。结论在AIH模型小鼠肺脏中不同的时间点上出现了IL-17A和24p3的表达,两者可能在肺脏炎症的发生中发挥重要的作用。 Objective To study the expression of IL-17A and 24p3 in the lung of autoimmune hepatitis （AIH） mouse. Methods The AIH animal model was inducing by ConA. The expression of IL-17A and 24p3 mRNA in the lung were detected by RT-PCR. The infiltration of lymphocyte was measured by HE. Results It was found that IL-17A was expressed in the lung of AIH mouse at different degrees and the expression of 24p3 was higher than that of IL-17A and reached the highest at 12h. The inflammation of lung appeared at 8h and was the most serious at 72h. Conclusion IL- 147A and 24p3 are expressed in the lung of AIH mouse at different times, which may be a significant role in the inflammation of lung.
作者 宋桂芹 刘康 梁素华 刘云 曾梅 申跃武 王婉
机构地区 川北医学院生物教研室 川北医学院第二临床医学院组织工程与干细胞研究所
出处 《西部医学》 2010年第4期591-594,共4页 Medical Journal of West China
基金 四川省教育厅青年基金项目(08zb073) 川北医学院苗圃基金(编号:基-03)
关键词 自身免疫性肝炎 IL-17A 24p3 Autoimmune hepatitis IL-17A 24p3
分类号 Q75 [生物学—分子生物学]
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参考文献11

  • 1Bonder CS,Ajuebor MN,Zbytnuik LD,et al.Essential Role for Neutrophil Recruitment to the Liver in Concanavalin A-In-duced Hepatitis[J].J Immunol,2004,172(1),45-53.
  • 2Park H,Li Z,Yang XO,Chang SH,et al.A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleu-kin 17[J].Nat Immunol,2005,6(11),1133-1141.
  • 3Mvoseley TA,Haudenschild DR,Rose L,et al.Interleukin-17 family and IL-17 receptors[J].Cytokine Growth Factor Rev,2003,14(2),155-174.
  • 4Chabaud M,Lubberts E,Joosten L,et al.IL-17 derived from juxta-articular bone and synovium contributes to joint degradation in rheumatoid arthritis[J].Arthritis Res,2001,3(3):168-177.
  • 5Shen F,Hu Z,Goswami J,et al.Identification of common transcriptional regulatory elements in interleukin-17 target genes[J].J Biol Chem,2006,281 (34),24138-24148.
  • 6Nilsen-Hamilton M,Hamilton RT,Adams GA,et al.Rapid selective stimulation by growth factors of the incorporation by BALB/C 3T3 cells of[35S]methionine into a glycoprotein and five superinducible proteins[J].Biochem Biophys Res Com-mun.,1982,108(1):158-166.
  • 7宋桂芹,何波,王琛琛,梁素华,刘康,刘全胜.小鼠分娩前后子宫IL-17A及24p3表达变化的研究[J].四川大学学报（自然科学版）,2008,45(3):681-686. 被引量：1
  • 8Devireddy LR,Teodoro JG,Richard FA,et al.Induction of ap-optosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation[J].Science,2001,293,829-834.
  • 9何波,高闻达,宋桂芹,王琛琛,杨明理,刘全胜.24p3及白细胞介素17A在自身免疫性肝炎中的表达[J].中华肝脏病杂志,2007,15(9):709-710. 被引量：4
  • 10Lapierre P.Beland K,Alvarez F.Pathogenesis of autoimm une hepatitis,from break of tolerance to immune-mediated hepato-cyte apoptosis[J].Transl Res,2007,149,107-113.

二级参考文献28

  • 1罗克燕,王豫黔.促炎性细胞因子IL-8、TNF-α与分娩发动[J].遵义医学院学报,2005,28(2):185-188. 被引量：1
  • 2Harrington LE, Hatton RD, Mangan PR, et al. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol, 2005, 6:1123-1132.
  • 3Park H, Li Z, Yang XO, et al. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol, 2005, 6:1133-1141.
  • 4Cua D J, Sherlock J, Chen Y, et al. Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature, 2003, 421: 744-748.
  • 5Shen F, Hu Z, Goswami J, et al. Identification of common transcriptional regulatory elements in interleukin-17 target genes. J Biol Chem, 2006, 281: 24138-24148.
  • 6Harrington L E, Robin D H, Paul R M, et al. Interleukin 17-producing CD4 + effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages [J]. Nat Immunol, 2005, 6(11): 1123.
  • 7Park H, Li Z X. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17 [J]. Nat Immunol, 2005, 6(11): 1133.
  • 8Mvoseley T A, Haudenschild D R, Rose L, et al. Interleukin-17 family and IL-17 receptors [J]. Cytokine Growth Factor Rev, 2003, 14(2) : 155.
  • 9Rouvler E, Luciani M, Mattei M, et al. CTLA-8, Cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene [J]. J Immunol, 1993, 150 (12) : 5445.
  • 10Chabaud M, Lubberts E, Joosten L, et al. IL-17 derived from juxta-articular bone and synovium contributes to joint degradation in rheumatoid arthritis [J]. Arthritis Res, 2001, 3(3) : 168.

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西部医学
2010年 第4期

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