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应用曲线拟合方法求解单克隆抗体亲和常数中OD_(100) 被引量：2

Application of curve-fitting method in measurement of OD_(100)
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摘要 目的采用曲线拟合方案测定单克隆抗体亲和常数计算中的OD100。方法以测定抗苏丹红-1单克隆抗体亲和常数为例,非竞争ELISA法测定OD值,经曲线回归拟合结合反应的曲线模型及其方程,依据方程计算OD100值。结果根据幂曲线模型计算的抗苏丹红-1单抗亲和常数为K=8.19×108l/mol。结论应用统计学分析方法拟合的曲线模型,科学可靠地测定了OD100,为单克隆抗体亲和常数的计算提供了科学、合理的测量基础。 Objective To calculate ODloo in measurement of affinity constant of monoclonal antibody. Methods The affinity constant of monoelonal antibodies against sudan-1 was determined by noncompetitive enzyme immunoassay with curve-fitting method. Results Power model was fitted based on scatter diagram of the binding reaction of antigen and antibody. Conclusion OD100 was determined credibly by application of statistical methods which provided a rational measurement for calculating affinity constant of monoclonal antibody.
作者 刘彦君 谭婧 章崇杰 宣景秀 魏大鹏
机构地区 四川大学华西基础与法医学院免疫学教研室 四川大学华西公共卫生学院
出处 《西部医学》 2010年第4期599-602,共4页 Medical Journal of West China
关键词 亲和常数 OD100 曲线拟合 模型 单克隆抗体 Affinity constant OD100 Curve-fitting Model Monoclonal antibody
分类号 R730.5 [医药卫生—肿瘤]
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参考文献13

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二级参考文献6

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二级引证文献7

西部医学
2010年 第4期

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