外周伤害性痛刺激对大鼠背海马神经元的影响

INFLUENCE OF PERIPHERAL NOXIOUS STIMULATION ON RAT HIPPOCAMPAL NEURONS

为了探讨海马在痛觉调控中的作用，本文应用FOS免疫组化技术结合镇痛药物分析了大鼠对化学性痛刺激的反应。结果表明：将5％福尔马林（100μl）向左前爪掌心皮下注射1h后，双侧海马出现大量的FOS免疫反应阳性神经元；吗啡（5．0mg／kg）预处理10min后再进行福尔马林注射，大鼠双侧背海马内的FOS免疫反应阳性神经元数量显著减少；吗啡（10．0mg／kg）预处理组大鼠，其背海马内的FOS免疫反应阳性神经元数量进一步减少；而纳洛酮在一定程度上具有翻转吗啡的作用。结果提示，海马参与了痛觉调控机制，特别是痛觉情绪的调控机制。

Objective: To study the role of hippocampus in regulation of pain. Methods: FOS immunocytochemical method was used to analyze the effect of chemical pain stimulation on rat, as well as the influence of analgesia. Results: (1)Large amount of FOS positive neurons were found in bilateral dorsal hippocampal formation. (2)The number of FOS positive neurons were significantly reduced by pretreatment with morphine (5 mg/kg or 10 mg/kg). But the effect of morphine was reversed by copretreatment with naloxone. Conclusions: Hippocampal formation participated in the regulation of formalin induced pain, especially pain related emotional activities.