CD_4^+CD_(25)^+CD_(127)^(low)调节性T细胞及相关细胞因子在儿童再障发病中的作用

Roles of CD_4^+CD_(25)^+CD_(low)^(127) regulatory T cells and related cytokine in children with aplastic anemia

目的探讨CD4+CD2+5CD127low调节性T细胞及转化生长因子-β1(TGF-β1)在再障患儿外周血中的表达及临床意义。方法采用流式细胞术分别检测21例慢性再障(CAA)、9例急性再障(SAA)和15例正常健康儿童外周血白细胞,并用酶联免疫吸附法检测其外周血TGF-β1表达水平。结果外周血中CD4+T细胞百分比、CD4+CD2+5、CD4+CD2+5CD127low调节性T细胞占CD4+T细胞百分比及血清TGF-β1表达水平再障患儿均低于正常健康儿童(P均<0.05),SAA低于CAA(P均<0.05);再障患儿CD4+CD2+5CD127low调节性T细胞占CD4+T细胞百分比与TGF-β1的表达水平无明显相关性。结论再障患儿外周血调节性T细胞比例减少、血清TGF-β1表达水平减低可能参与了再障的发病。

Objective To explore the levels of CD4^＋CD25^＋CD127low regulatory T cells （Tregs） and TGF-β1 in peripheral blood of aplastic anemia （AA） in children, and to investigate the role of Tregs in the pathogenesis of aplastic anemia. Methods A total of 21 patients with chronic aplastic anemia （CAA）, 9 patients with acute aplastic anemia （SAA） and CD4^＋CD25^＋CD127low T ceils in CD4＋ T cells was evalu-15 normal healthy children were included in this study. The proportion of CD4＋CD25^＋CD127^low ated by flow cytometric analysis. TGF-β1 was measured by ELISA. Results The percentage of CD4＋ T cells, CD4＋CD25^＋ T cells. Tregs in peripheral blood and serum TGF-β1 expression in patients with AA were lower than those in the normal control （all P 〈 0.05）. The percentage mentioned above in SAA was lower than that in CAA （all P 〈 0.05 ）. Tregs percentage and the level of TGF-β1 in children with AA were no significant correlation. Conclusions The low percentage of regulatory T cells and the low level of TGF-β1 in children may be involved in pathophysiology of AA.