二茂铁甲酸分子印迹聚合物的制备、识别机理和结合特性

Preparation,Mechanism of Recognition and Binding Characteristics of Ferrocenecarboxylic Acid Imprinted Polymers

以二茂铁甲酸(FCA)为模板,选用不同的功能单体制备了一系列分子印迹聚合物,用平衡结合实验考察了它们对模板分子的结合性能。结果表明,以甲基丙烯酸为功能单体制得的印迹聚合物P1对模板分子有很好的选择性,特异性吸附量ΔCP为23.18μmol/g,印迹因子IF为2.33,竞争性结合实验结果表明,P1可以将模板分子从结构类似物中分离出来。Scatchard方程研究表明,在研究的浓度范围内聚合物中形成了一类等价的结合位点,其对模板分子的平衡离解常数K=1.94 mmol/L,最大表观结合量Cpmax=92.33μmol/g。研究还表明,FCA的羧基是在聚合物的孔穴中产生识别位点的功能基,模板分子上的羧基与MAA的羧基形成双重氢键作用是分子识别的主要作用力。

A series of ferrocenecarboxylic acid imprinted polymers from different functional monomers were prepared and their adsorption properties for the template were evaluated by equilibrium binding experiments.The results indicate that the imprinted polymer P_1,prepared with methacrylic acid as the functional monomer, exhibited a higher selectivity for ferrocenecarboxylic acid.The specific binding capacity（ΔC_P） and imprinting factor（IF） of P_1 are 23.18 μmol/g and 2.33,respectively.The results of competitive binding experiments show that P_1 can separate the template from its structural analogues.Scatchard analysis demonstrates that one equal class of binding sites was formed in the imprinted polymer in the studied concentration range.The dissociation constant and the apparent maximum number of the binding sites are 1.94 mmol/L and 92.33 μmol/g,respectively.The study suggests that the carboxyl of the template is the function group responsible for the formation of complementary interacting site in the polymer P_1,and the double hydrogen bonds formed between the carboxyl groups of ferrocenecarboxylic acid and methacrylic acid are the main interactions in the recognition process.