FNA的抑制作用及反硝化过程的交叉影响

Inhibitory Effect of Free Nitrous Acid and Cross Inhibition in Denitrification

亚硝酸盐和硝酸盐通常被认为对反硝化细菌的代谢有抑制作用.通过大量批式试验,对不同浓度亚硝酸盐在不同pH条件下对硝酸盐还原的抑制作用及2种电子受体之间的交叉影响做了研究.结果表明,硝酸盐还原与游离亚硝酸(FNA)有显著的相关关系,FNA而非亚硝酸盐是硝酸盐还原的真正抑制剂.FNA浓度为0.01～0.025mg·L-1时硝酸盐还原能力受抑制程度为60%,当FNA浓度>0.2mg·L-1时,硝酸盐还原反应被完全抑制.此外,污泥亚硝酸盐还原能力也受FNA抑制,当FNA浓度由0.01mg·L-1增至0.2mg·L-1,亚硝酸盐还原能力下降80%.研究还发现,亚硝酸盐还原受硝酸盐抑制影响很小,不同浓度抑制剂下还原能力恢复达90%以上.相反,硝酸盐还原能力仅恢复3.04%～72.54%,且恢复程度主要取决于抑制剂投加量,而受抑制时间和抑制剂投加方式影响较小.

Nitrite and nitrate have been generally recognized to have an inhibitory effect on metabolism of denitrifiers. The nitrite inhibition on nitrate reduction under various pH conditions and the cross effect of the both electron acceptors on each other were investigated through a series of batch tests by using a biological nutrient removal （ BNR） sludge. The results showed that the nitrate reduction activity had a much stronger relationship with the free nitrous acid （FNA） than that of nitrite concentration,suggesting that FNA,rather than nitrite is likely the actual inhibitor on nitrate reduction. Sixty percent inhibition of nitrate reduction was observed at an FNA concentration of 0. 01-0. 025 mg·L^-1 ,while total inhibition occurred when the FNA concentration was greater than 0. 2 mg·L^-1. Furthermore,nitrite reduction by the BNR sludge was also found to be inhibited by HNO2. The reduction rate decreased by approximately 80% when the FNA concentration was increased from 0. 01 mg·L^-1 to 0. 2 mg·L^-1. The inhibitory effect of nitrate on nitrite reduction was found to be insignificant,with the most recovery rates under different nitrate concentrations larger than 90%. However,the nitrate reduction rate was observed to recover only 3. 04% -72. 54%. The recovery rate from inhibition was independent of the duration of the inhibition and the feeding mode of inhibitor,but strongly dependent on the concentration of inhibitor the biomass was exposed to during the inhibition period.