基于元分析的大豆胞囊线虫抗性QTL的整合

Integration of QTLs related to soybean cyst nematode resistance based on meta-analysis

以2004年发布的大豆公共遗传图谱soymap2为参考图谱,共搜集整理了自1994年以来已报道的与抗大豆胞囊线虫相关的151个QTLs,通过BioMercator2.1和公共标记将相关QTLs映射整合到大豆公共遗传连锁图谱soymap2上,并利用元分析技术发掘"真实"QTL。本文共发掘出与抗大豆胞囊线虫1、2、3、4、5和14号生理小种相关的16个"真实"QTL,其中有四个位点的图距小于1.5cM,一个位点的图距小于5cM,分布于A2、E和G连锁群,其中G连锁群上5.11cM处的定位区间包含已报道的Rhg1位点;发现在G连锁群上有一个定位区间控制1,4号生理小种,在B1连锁群上有一个定位区间控制2,5号生理小种,可见这些位点具有兼抗性。

According to soybean public map soymap2 （published in 2004）,151 QTLs related to soybean cyst nematode resistance were collected from markers reported since 1994. They were integrated and projected to the reference soymap2 using BioMercator2.1 software. 16 ＂real＂ QTLs and their corresponding markers were obtained using meta-analysis method to narrow down the confidence interval. The ＂real＂ QTLs included markers related cyst nematode resistance to race 1,2,3,4,5,and 14. Part of them distributed on A2,E,and G linkage groups （LGs）. Four of them had intervals less than 1.5 cM and one of them less than 5 cM. Rhg1,a previously reported locus,was found on LG G with 5.11 cM. Two of them located on LG B1 which related to race 2 and 5,another two on LG G related to race1 and 4. These results indicated double resistances to soybean cyst nematode and offered a basis for gene mining or molecular breeding in soybean.